The Study of LasI and AlgD genes frequency and Multidrug Resistance (MDR) in Pseudomonads isolated from infectious samples of Fasa medical centers

Background and Objective: Pseudomonas aeruginosa is one of the most important pathogenic bacteria causing infectious diseases that include resistance to many antibiotics. In addition, existence of LasIand AlgD gene contributes to pathogenicity of this bacterium. This study is aimed to investigate the frequency of mentioned genes, ‌antibiotic resistance and a significant relationship between them. Materials and Methods: This descriptive cross-sectional study was performed on 91 isolated Pseudomonas from Fasa medical centers. After completing biochemical and microbiological tests, all strains were evaluated by the PCR method in terms of LasIand AlgD genes existence, and antibiotic susceptibility test was performed by disk diffusion and MIC methods. Statistical analysis was performed using SPSS 20 software. Results: Among 91 isolated samples, (59.34%) 54 samples of Pseudomonas aeruginosa and (40.66%) 37 samples of other species were detected. The results of drug resistance pattern determination for both groups showed that highest resistance to Cefepime has been established. 40 isolated Pseudomonas aeruginosa and 30 isolated samples of other species showed a MIC test relative to Cefepime. All isolated samples had AlgD gene. Conclusion: The results of this study revealed high resistance to different antibiotics among Pseudomonas aeruginosa strains and other strains. Quorum sensing genes and alginate generator genes with antibiotic resistance genes can increase the severity of involvement in this group of bacteria.